Analysis Note

ControlMouse E13-E14 brain tissue lysate

Application

Anti-OL-protocadherin Antibody, clone 5G10 is an antibody against OL-protocadherin for use in WB, IC, IH.

Immunofluorescence Analysis: The antibody has been shown to detect OL-protocadherin in IF. (Nakao, 2008).

Immunohistochemistry Analysis: The antibody has been shown to detect OL-protocadherin in IH. (Aoki, 2003).

Research CategoryCell StructureNeuroscience

Research Sub CategoryAdhesion (CAMs)Synapse & Synaptic Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

OL-protocadherin (OL-pc, protocadherin 10) is a transmembrane protein and a member of the cadherin superfamily, which is characterized by its members' abilities to undergo interactions that are homophilic in nature via their extracellular domain. Several protocadherins have been observed within the nervous system, and a few have been shown to localize at synapses. With OL-pc, the homophilic binding has been shown to stimulate cell aggregation when the protein is introduced into cadherin-deficient cells. Although the function of OL-protocadherin has yet to be elucidated, studies have shown OL-pc to be necessary to axonal normal projection through the ventral telencephalon, where OL-pc shows highest expression at the striatal region.

Immunogen

Epitope: Cytoplasmic domain

GST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse OL-protocadherin.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in mouse E13-E14 brain tissue lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected OL-protocadherin in 10 µg of mouse E13-E14 brain tissue lysate.

Specificity

The antibody recognizes the cytoplasmic domain of OL-protocadherin.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 130 kDa observed